Coding

Part:BBa_K2382015:Design

Designed by: HO-LO HUANG   Group: iGEM17_CSMU_NCHU_Taiwan   (2017-10-26)


Thioredoxin-FGD fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1030
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1079
    Illegal BamHI site found at 367
    Illegal XhoI site found at 373
    Illegal XhoI site found at 1345
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 739
    Illegal NgoMIV site found at 937
    Illegal AgeI site found at 703
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Theoretically, thioredoxin (BBa_K2382004)could help protein folding in the cell, thus,we combined the FGD(BBa_K2382002) and thioredoxin together to make a new fusion protein. The two glycine between the linker and FGD are designed for a better flexibility.

Source

thioredoxin Escherichia coli str. K-12 substr. MG1655 ( NC_000913.3 )

FGD Mycobacterium smegmatis str. MC2 155 (AAC38338.1)